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Ama-pipette ayizinsiza ezibalulekile ekwenzeni ucwaningo lwezokwelapha, njengoba avumela ukukala nokudlulisa amanzi ngokunembile nangokwethembeka. Ukufakwa kwalesi sikhathi sohlobo lwensiza kubalulekile cishe kuzo zonke izivivinyo nezihlolo lapho kudingeka khona ivolumu encane yamanzi. Le ndatshana igxile emsebenzini wama-pipette ocwaningweni lwezokwelapha, ichaza izinhlobo zama-pipette, izindawo zokusebenza, kanye nokusetshenziswa kwazo okufanele. Ngaphezu kwalokho, izindlela ezisha ezithuthukisa umkhawulo wokukhiqiza ezinqubweni zocwaningo zizogqamiswa.
Mayelana nocwaningo lwezokwelapha, akukwaziwa ukuthi kubaluleke kangakanani ama-pipette. Yizinsiza ezibalulekile emsebenzini omncane okuhlanganisa: ukulungiselela amasampula, ukwenza izivivinyo noma ukwenza umsebenzi wokunciphisa. Ukukhetha i-pipette efanele kubaluleke ngokufanayo. Ngoba, ngakolunye uhlangothi, ukukhetha kahle i-pipette kunezimpempe ezicacile nezinkulu emthethweni wokuphindaphinda imiphumela yokuhlola ebikwe. Ngakolunye uhlangothi, kukhona inani elikhulu lama-pipette ahlukene emhlabeni. Okokuqala: ama-pipette okukhishwa komoya, i-pipette yokukhishwa okuhle, kanye ne-pipette kagesi, ngayinye ihloselwe ukusetshenziswa okuthile kanye nezinto eziphilayo.
Uhlobo oluthile lwepipette olwaziwa njenge-air displacement pipette, lujwayelekile emalabhorethri amaningi futhi ngokuvamile lusetshenziswa kakhulu. Le pipette isebenza ngokwakha i-vacuum usebenzisa i-syringe futhi ikhipha liquid edingekayo ibe yitip elisetshenziswayo. Lezi pipettes zisebenza kahle nezixazululo ezinomswakama; ngakho-ke, zikhona kakhulu emisebenzini ye-molecular biology kanye ne-biochemistry. Nokho, i-positive displacement pipette isebenza ngokusebenzisa indlela yokususa enepiston esebenzayo kwiLiquid engase ibe neFoamy kakhulu noma eneviscous. Ama-electronic pipettes ahlanganisa kakhulu izinga eliphezulu lokunemba lapho ahlelwa khona okwenza kube kufanelekile kumaphrothokholi ayinkimbinkimbi & izinhlelo zokusebenza eziphezulu.
Ukuze kufezwe imiphumela efunwayo, kubalulekile kakhulu ukwenza kahle indlela yokuphehla. Ukuqeqeshwa okufanele kumele kunikezwe abacwaningi mayelana nokusetshenziswa okufanele kwepipette njengokuthi ithiphu efanele, ukuwasha ngaphambi kokusebenzisa amathiphu, ijubane lokuphehla, njll. Kubalulekile futhi ukuthembela ezicombeni zokmaintenance ezifanele ezizosiza ekubuyiselweni kabusha nasekugcineni ama-pipette ezingeni elidingekayo. Ekugcineni, ukufaka indlela efanele yokuphehla kungasiza ukuvimbela amaphutha, kuholele ekuthuthukiseni ikhwalithi jikelele yocwaningo.
Ukuguquka kobuchwepheshe kuletha ukuthuthukiswa okusha ekwakhiweni nasekusebenzeni kwepipette. Imikhiqizo emisha ifika emakethe, kuhlanganise neziphuzo ezihlakaniphile ezisebenzisa ama-sensor ahlanganisiwe nezinhlelo zokusebenza futhi zikwazi ukukala inqubo ye-pipetting. Lezi zithuthukisi akukhuphuli kuphela ukunemba kodwa futhi kuthuthukisa nezinqubo zomsebenzi zezimboni eziphambili kakhulu. Ukusetshenziswa okukhulayo kokwenza ngokuzenzakalelayo ezikhungweni zokuhlola kuthinta futhi ukwakhiwa kwepipette, ezisetshenziswa kakhulu manje ekwenzeni ukuhlolwa okukhulu nasekulungiseleleni amasampula.
Ekuphethweni, ama-pipette aphakathi kwezinsiza eziyisisekelo ezibalulekile ocwaningweni lwezokwelapha ngenxa yokunembile nokwethembeka ekuphathweni kwe-liquid. Abacwaningi baphakanyiswa ukukhetha i-pipette efanele ezinhlelweni ezithile bahlola izinhlobo ezahlukene. Ukuqhubeka phambili kwezobuchwepheshe bokuthuthukiswa kwama-pipette kungenzeka kuthuthukise ukusebenza kahle nokunembile ekusebenzeni kocwaningo ngakho-ke kwenza kube usizo ezindleleni zokusebenza zelebhu zanamuhla. Ngokuhambisana nokukhula kwalesi sikhala socwaningo lwezokwelapha, kuzoba khona ubudlelwano phakathi kwama-pipette nokuthuthukiswa kocwaningo obuzohlala buhlangene.