Recently, the diagnostic field has been revolutionized by the introduction of saliva collection tubes in diagnostic testing. Thanks to these devices, there is no longer any need to invasive mode of sampling as these devices are easy and efficient in collecting biological samples. This article discusses how diagnostics are becoming better with the help of saliva collection tubes, their benefits, and what the future may hold for such devices in the field of testing. As the name explains, saliva collection tubes are meant to enhancing collection, preservation, and transport of saliva. Saliva sampling is simple and painless unlike blood sampling which is difficult and painful. This is particularly useful in cases of children (pediatrics) who may be terrified of the needle. In addition, saliva samples can be taken from various places, including the patients’ homes, which offers convenience to patients and decreases the load on healthcare facilities. However, it was found out that one of the major benefits is accurate and reliable diagnostic information especially using saliva collection tubes. It is known that saliva contains a number of biomarkers, through which the level of different health indicators can be made, such as: hormonal levels, infectious diseases, and even genetic predisposition. Newer studies have indicated that comparable results can be obtained through saliva based testing as well which makes it very potent in many diagnostic cases. It is also worth mentioning that, the increasing use of saliva collection tubes can be attributed to improvement in technology. The contemporary saliva collection tubes are designed with special preservatives that will allow the samples to be kept for a long time without causing any degradation of the biomarker. Also, the use of digital health technologies permits the collection, storage, and analysis of saliva samples in real time which will enhance the prevention and treatment of disease through targeted interventions. Hence, with the increase in the number of patients requiring such low-invasive or non-invasive methods of diagnosis, the market penetration of saliva collection tubes in future is estimated to be substantial. These instruments are being appreciated by the clinicians as they improve the patients’ compliance and even help in the quality of life. In addition, with the growing interest in the diagnostic capabilities of saliva, a new generation of saliva based tests should be on the agenda which would dramatically change the way in which the disease is diagnosed and managed. To conclude, saliva collection tubes are not merely a fad; they are the future of diagnostic testing expansion. There is an opportunity to improve patient care and the prospects of detecting illness in its early stages with a non-invasive and efficient and dependable way of sample collection. The future looks bright with further advancements in this field that will enhance the use of saliva in diagnostics even more and it will become an integral part of diagnostics for the medical profession around the world.